Molecular and immunohistochemical diagnosis of Francisella noatunensis subsp. orientalis from formalin-fixed, paraffin-embedded tissues
সংরক্ষণ করুন:
| লেখক: | , , , , , , , , , , , , , |
|---|---|
| বিন্যাস: | artículo |
| প্রকাশনার তারিখ: | 2012 |
| বিবরন: | Members of the genus Francisella (viz., F. noatunensis subsp. orientalis [Fno] and F. noatunensis subsp. noatunensis) have been described as causative agents of chronic granulomatous and pyogranulomatous lesions in wild and cultured fish species. In the present study, 68 archived formalin-fixed, paraffin-embedded (FFPE) tissues from several fish species, collected at different geographical locations from 2000 to 2011, were analyzed using a real-time polymerase chain reaction assay for the detection of the Fno intracellular growth loci C (iglC) gene and by immunohistochemistry for the demonstration of Fno antigens. The results revealed a high correlation between these 2 diagnostic techniques validating their use for the diagnosis of Fno infection in archived FFPE tissues and confirming the presence of Fno in fish species from the Cari y years of the present century. |
| দেশ: | Repositorio UNA |
| প্রতিষ্ঠান: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| ভাষা: | Inglés |
| OAI Identifier: | oai:null:11056/18041 |
| অনলাইন ব্যবহার করুন: | http://jvdi.sagepub.com http://hdl.handle.net/11056/18041 |
| মুখ্য শব্দ: | PECES MARINOS FRANCISELLA REACCION DE CADENA DE LA POLIMERASA REAL-TIME POLYMERASE CHAIN REACTION MARINE FISH |