First isolation and molecular characterization of Ehrlichia canis in Costa Rica, Central America
محفوظ في:
| المؤلفون: | , , , , , , , |
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| التنسيق: | artículo |
| تاريخ النشر: | 2010 |
| الوصف: | The present study investigated Ehrlichia species in blood samples from dogs suspected of clinical ehrlichiosis, using molecular and isolation techniques in cell culture. From a total of 310 canine blood samples analyzed by 16S rRNA nested PCR, 148 (47.7%) were positive for Ehrlichia canis. DNA from Ehrlichia chaffeensis or Ehrlichia ewingii was not detected in any sample using species-specific primers in separated reactions. Leukocytes from five PCR-positive dogs were inoculated into DH82 cells; successful isolation of E. canis was obtained in four samples. Partial sequence of the dsb gene of eight canine blood samples (including the five samples for in vitro isolation) was obtained by PCR and their analyses through BLAST showed 100% of identity with the corresponding sequence of E. canis in GenBank. This study represents the first molecular diagnosis, isolation, and molecular characterization of E. canis in dogs from Costa Rica. |
| البلد: | Repositorio UNA |
| المؤسسة: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| اللغة: | Inglés |
| OAI Identifier: | oai:null:11056/22968 |
| الوصول للمادة أونلاين: | http://hdl.handle.net/11056/22968 |
| كلمة مفتاحية: | EHRLICHIA CANIS COSTA RICA PERRO DIAGNOSTICO (MEDICINA VETERINARIA) EPIDEMIOLOGIA VETERINARIA ENFERMEDADES INFECCIOSAS EHRLICHIA DOG VETERINARY EPIDEMIOLOGY INFECTIOUS DISEASES |