Monitoring female reproductive function by measurement of fecal estrogen and progesterone metabolites in the white-faced saki (Pithecia pithecia)
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| Autoři: | , , , , |
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| Médium: | artículo |
| Datum vydání: | 1994 |
| Popis: | A simple method for extracting and measuring ovarian steroids in feces is applied to the ovarian cycle, pregnancy, parturition, and period of lacta tional amenorrhea in Pithecia pithecia. Small amounts of wet, unmixed feces were combined with a modified phosphate buffer, shaken, centri fuged, and decanted, and the supernatant was directly measured for es trogen and progesterone metabolites by enzyme immunoassays. Urinary estrogen and progesterone metabolite measurements were compared to paired fecal measurements to determine the degree to which fecal hor mone levels detected the same ovarian events as urinary measurements. The correlation coefficients for the relationship between urinary and fecal hormones for individual animals studied (n = 5) were found to be statis tically significant in every case except one sexually immature animal. The application of the method presented here demonstrates that simple solu bilization and non-radiometric measurement of ovarian steroids excreted in feces reliably reflect reproductive events in Pithecia pithecia. 0 1994 Wiley-Liss, Inc. |
| Země: | Repositorio UNA |
| Instituce: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Jazyk: | Inglés |
| OAI Identifier: | oai:null:11056/23676 |
| On-line přístup: | http://hdl.handle.net/11056/23676 https://doi.org/10.1002/ajp.1350320203 |
| Klíčové slovo: | CICLO ESTRAL REPRODUCCIÓN ANIMAL ESTROUS CYCLE OVARIO ANIMAL REPRODUCTION |