Weighted gene co-expression network analysis (WGCNA) of iPSC Generated from Patients with Schizophrenia
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| Autores: | , , |
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| פורמט: | contribución de congreso |
| Fecha de Publicación: | 2019 |
| תיאור: | Aims: Human induced pluripotent stem cells (hiPSC) have provided a new way of studying Schizophrenia (SZ), by allowing the establishment of cellular models accounting for the patient genetic background. Here we conducted an exploratory RNA-sequencing profiling study of different cell lines derived from hiPSCs generated from somatic cells of subjects from the population isolate of the Central Valley of Costa Rica (CVCR) Methods: RNA from these cell were then sequenced using Illumina HiSeqTM2500. Raw count data measured 48162 transcripts across all samples. The 20000 more expressed genes were selected. Normalized values were used as input for weighted gene coexpression network analysis (WGCNA). Differential expression of MEs (module eigengene) comparing healthy controls and patients with schizophrenia across all cell types were performed. Results and Conclusions: On total 4 cell lines (LCL, hiPSC, NPC and cortical neurons) of 6 healthy controls (HC) and 7 SZ patients from the CVCR were included on the WGCNA analysis. Biweight midcorrelation was used to define the coexpression similarity. Differential expression of MEs were observed on relation to phenotype and cell type. Mental disorders have a major impact on individual health, impairing both personal and professional activities, being therefore an important burden for society. Agreat advantage of using iPS-derived cells is that the effect of outside environmental influences, such as use of medications, is removed and only the effects of genetic composition, which is unchanged by transformation, are left. Our study use WGCNA to establish blocks of gene expression on a hiPSC cellular model of SZ related to phenotype. Several modules were significantly correlated with cell type study. When analyzing only hiPSC-neurons, modules correlated with the disease were identified. |
| País: | Kérwá |
| מוסד: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| שפה: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/103610 |
| גישה מקוונת: | https://hdl.handle.net/10669/103610 https://doi.org/10.1016/j.jad.2018.10.353 |
| מילת מפתח: | induced pluripotent stem cells schizophrenia RNA sequencing gene coexpression networks |