Isolation of two basic phospholipases A2 from Bothrops diporus snake venom: comparative characterization and synergism between Asp49 and Lys49 variants

 

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Autores: Bustillo, Soledad, Fernández Ulate, Julián, Chaves Araya, Stephanie, Angulo Ugalde, Yamileth, Leiva, Laura C., Lomonte, Bruno
Formato: artículo original
Fecha de Publicación:2019
Descripción:Bothrops diporus, previously considered a subspecies of the B. neuwiedi complex, is a medically relevant viperid in Northeastern Argentina. The venom of this species causes local tissue damage characterized by myonecrosis, hemorrhage, blistering, and edema. In the present study, two basic phospholipases A2 (PLA2-I and PLA2-II) were isolated from this venom, and their pathological effects upon murine skeletal muscle and myogenic cells in culture were analyzed. Partial amino acid sequencing showed that PLA2-I and PLA2-II are Asp49 and Lys49 PLA2s, respectively. In agreement with this, PLA2-I showed PLA2 activity, whereas PLA2-II did not. Functional assays revealed differences in their myotoxicity, cytotoxicity, and anti-adhesion activity, and in the ability to inhibit cell migration, all of which were greater for the Lys49 variant. Native electrophoresis showed that PLA2-I was less basic than PLA2-II. The two proteins act synergistically to affect the integrity of C2C12 myogenic cells, providing a further example of the concerted action of coexisting snake venom components. PLA2-I and PLA2-II, together with additional basic PLA2s revealed by RP-HPLC, probably play an important role in myonecrosis after envenomation by B. diporus.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/80379
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0041010119304076?via%3Dihub
https://hdl.handle.net/10669/80379
Palabra clave:Bothrops diporus
Phospholipase A2
Synergism
Myotoxicity
Cytotoxicity
snake venom