Dermonecrosis caused by a spitting cobra snakebite results from toxin potentiation and is prevented by the repurposed drug varespladib

 

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Nhiều tác giả: Bartlett, Keirah E., Hall, Steven Robert, Rasmussen, Sean A., Crittenden, Edouard, Dawson, Charlotte A., Albulescu, Laura Oana, Laprade, William Michael, Harrison, Robert A., Saviola, Anthony J., Modahl, Cassandra M., Jenkins, Timothy Patrick, Wilkinson, Mark C., Gutiérrez, José María, Casewell, Nicholas R.
Định dạng: artículo original
Ngày xuất bản:2024
Miêu tả:Snakebite envenoming is a neglected tropical disease that causes substantial mortality and morbidity globally. The venom of African spitting cobras often causes permanent injury via tissue-destructive dermonecrosis at the bite site, which is ineffectively treated by current antivenoms. To address this therapeutic gap, we identified the aetiological venom toxins responsible for causing local dermonecrosis. While cytotoxic three-finger toxins were primarily responsible for causing spitting cobra cytotoxicity in cultured keratinocytes, their potentiation by phospholipases A2 toxins was essential to cause dermonecrosis in vivo. This evidence of probable toxin synergism suggests that a single toxin-family inhibiting drug could prevent local envenoming. We show that local injection with the repurposed phospholipase A2-inhibiting drug varespladib significantly prevents local tissue damage caused by several spitting cobra venoms in murine models of envenoming. Our findings therefore provide a new therapeutic strategy to more effectively prevent life-changing morbidity caused by snakebite in rural Africa.
Quốc gia:Kérwá
Tổ chức giáo dục:Universidad de Costa Rica
Repositorio:Kérwá
Ngôn ngữ:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/104205
Truy cập trực tuyến:https://hdl.handle.net/10669/104205
https://doi.org/10.1073/pnas.2315597121
Từ khóa:venoms
toxins
snakebite envenoming
neglected tropical diseases
drug repurposing