Reverse-transcription loop-mediated isothermal amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy
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| Autores: | , , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 2022 |
| Descripción: | Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints. Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions. Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/91685 |
| Acceso en línea: | https://hdl.handle.net/10669/91685 |
| Palabra clave: | emerging economies molecular testing SARS-CoV-2 saliva loop mediated isothermal amplification economías emergentes amplificación isotérmica mediada por bucle COVID-19 pandemic pruebas moleculares low cost testing large-scale testing |