In vitro activity of BaH1, the main hemorrhagic toxin of Bothrops asper snake venom on bovine endothelial cells

 

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Detalles Bibliográficos
Autores: Borkow, Gadi, Gutiérrez, José María, Ovadia, Michael
Formato: artículo original
Fecha de Publicación:1995
Descripción:Incubation of BaH1, the main hemorrhagic toxin purified from the venom of Bothrops asper, with endothelial cells caused the appearance of spaces among the cells. This effect became more noticeable with increasing hemorrhagin concentration and longer incubation time. Later, the cells became rounded and detached from the substrate into the medium. Augmentation of Trypan blue did not stain the detached cells, indicating their viability. Moreover, after washing the floating cells from the toxin they could be recultivated: they again spread on the substrate and proliferated, demonstrating that BaHl is not directly cytotoxic to the endothelial cells
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/29242
Acceso en línea:http://www.sciencedirect.com/science/article/pii/004101019500078Z
https://hdl.handle.net/10669/29242
Palabra clave:Animals
Bothrops
Cattle
Crotalid Venoms
Endopeptidases
Endothelium
Endothelium, Vascular
In Vitro Techniques