Physicochemical and immunological effects of adjuvant formulations with snake venom antigens for immunization of horses for antivenom production
সংরক্ষণ করুন:
| লেখক: | , , , , , , , |
|---|---|
| বিন্যাস: | artículo original |
| প্রকাশনার তারিখ: | 2023 |
| বিবরন: | Enhancement of antivenom immune responses in horses through adjuvant technology improves antivenom production efficiency, but substantial local reactogenicity associated with some traditional veterinary adjuvants limits their usability. To explore modern adjuvant systems suitable for generating antivenom responses in horses, we first assessed their physicochemical compatibility with Bothrops asper snake venom. Liposome and nanoparticle aluminum adjuvants exhibited changes in particle size and phospholipid content after mixing with venom, whereas squalene emulsion-based adjuvants remained stable. Next, we evaluated serum antibody response magnitude and neutralization capacity in horses immunized with adjuvant-containing Echis ocellatus, Bitis arietans, Naja nigricollis, and Dendroaspis polylepis venom preparations. Whereas all tested adjuvants elicited significant neutralization capacity against the viperid venoms, the greatest antibody responses were generated by a squalene-in-water emulsion, thus representing a promising novel alternative for antivenom production. |
| দেশ: | Kérwá |
| প্রতিষ্ঠান: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| ভাষা: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104214 |
| অনলাইন ব্যবহার করুন: | https://www.sciencedirect.com/science/article/pii/S0041010123002155?via%3Dihub https://hdl.handle.net/10669/104214 https://doi.org/10.1016/j.toxicon.2023.107229 |
| মুখ্য শব্দ: | snakebite snake venom antivenom adjuvant formulation horse immunization adjuvant-antigen compatibility |