Skeletal muscle necrosis induced by a phospholipase A2 isolated from the venom of the coral snake Micrurus nigrocinctus nigrocinctus.

 

محفوظ في:
التفاصيل البيبلوغرافية
المؤلفون: Arroyo Gutiérrez, Olga, Rosso Julien, Jean Pierre, Vargas Rosso, Orietta, Gutiérrez, José María, Cerdas Fallas, Luis
التنسيق: artículo original
تاريخ النشر:1987
الوصف:1. The venom of the coral snake Micrurus nigrocinctus nigrocinctus was fractionated by reverse-phase high performance liquid chromatography and an acidic myotoxic phospholipase A2 was purified to homogeneity. 2. After intramuscular injection, the toxin induced rapid and drastic myonecrosis, as serum creatine kinase levels increased markedly, reaching their highest values by 1.5 hr. 3. Ultrastructural observations indicate that the plasma membrane was the first structure to be affected, with the presence of focal disruptions in its integrity. 4. Myofilaments were hypercontracted and formed dense clumps. Sarcoplasmic reticulum integrity was lost, as evidenced by the presence of many small vesicles in the cellular space. 5. Some mitochondria were swollen, whereas others contained dense intracristal spaces and flocculent densities. Moreover, some had only one membrane. 6. In conclusion, pathogenesis of myonecrosis induced by this phospholipase A2 is similar to that induced by crude Micrurus nigrocinctus nigrocinctus venom.
البلد:Kérwá
المؤسسة:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/29150
الوصول للمادة أونلاين:https://hdl.handle.net/10669/29150
كلمة مفتاحية:Animals
Elapid venom
Mice
Microscopy
Muscles
Drug Effects
Necrosis
Phospholipases A2
Snake venom