Detection of the Trp-2027-Cys Mutation in Fluazifop-P-butyl–resistant Itchgrass (Rottboellia cochinchinensis) using High-Resolution Melting Analysis (HRMA)
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| Autores: | , , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 2018 |
| Descripción: | Itchgrass [Rottboellia cochinchinensis (Lour.) Clayton] is recognized as one of the most noxious and troublesome annual weeds in tropical and subtropical regions. Acetyl-coenzyme A carboxylase (ACCase)-inhibiting herbicides have been frequently used for managing R. cochinchinensis POST in a variety of crops, resulting in evolved resistance to these herbicides. Recently, resistance to fluazifop-P-butyl has been demonstrated for this weed, as the result of a G-to-C single-nucleotide polymorphism (SNP) that leads to the Trp-2027-Cys substitution in the ACCase enzyme. This study was conducted to develop a high-resolution melting analysis (HRMA) for the detection of the mutation underlying the Trp-2027-Cys substitution. The HRMA assay allowed differentiating between fluazifop-P-butyl–resistant (C mutant) and susceptible (G wild type) R. cochinchinensis plants. HRMA accuracy was confirmed with DNA sequencing of the target-site mutation, and no false positives or negatives were observed. Our results illustrated how HRMA is effective detecting the Trp-2027-Cys substitution in an R. cochinchinensis resistance, and how this technique can be of great value for developing high-throughput programs for monitoring evolution and dispersion of target site–based herbicide resistance at large scales. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/85471 |
| Acceso en línea: | https://www.cambridge.org/core/journals/weed-science/article/detection-of-the-trp2027cys-mutation-in-fluazifoppbutylresistant-itchgrass-rottboellia-cochinchinensis-using-highresolution-melting-analysis-hrma/5A2322A6F6AEA0759A0B0141C8A59116 https://hdl.handle.net/10669/85471 |
| Palabra clave: | ACCase inhibitors Genotyping Grass weeds Resistance SNPs |