Isolation and Molecular Characterization of Acanthamoeba Strains from Dental Units in Costa Rica
Đã lưu trong:
| Nhiều tác giả: | , , , , , , |
|---|---|
| Định dạng: | artículo original |
| Ngày xuất bản: | 2015 |
| Miêu tả: | Free-living amoebae are protozoa widely distributed in nature, which can be found in a variety of environments. Four genera are recognized as causal agents of infections in humans and animals: Acanthamoeba, Naegleria, Balam uthia, and Sappinia. In this study, the presence of Acanthamoeba in dental units was determined and the isolates obtained were molecularly character ized; osmotolerance and thermotolerance assays were also performed to evaluate multiplication under these conditions, frequently associated with pathogenicity. The morphological analysis and partial sequencing of the 18S rDNA gene revealed the presence of Acanthamoeba genotype T4 in 14% of the units sampled. Osmotolerance and thermotolerance tests were positive for more than 80% of the isolates. Up to date, this is the first study that reports the detection, identification, and genotyping of Acanthamoeba isolated from dental units in Costa Rica and even in Latin-America. Further assays to determine the potential pathogenicity of these Acanthamoeba isolates are underway. |
| Quốc gia: | Kérwá |
| Tổ chức giáo dục: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Ngôn ngữ: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/88196 |
| Truy cập trực tuyến: | https://onlinelibrary.wiley.com/doi/10.1111/jeu.12229 https://hdl.handle.net/10669/88196 https://doi.org/10.1111/jeu.12229 |
| Từ khóa: | Free-living amoeba Identification Odontology Pathogenicity Water |