Snake venomics of monocled cobra (Naja kaouthia) and investigation of human IgG response against venom toxins
Guardado en:
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo original |
| Fecha de Publicación: | 2015 |
| Descripción: | The venom proteome of the monocled cobra, Naja kaouthia, from Thailand, was characterized by RPHPLC, SDS-PAGE, and MALDI-TOF-TOF analyses, yielding 38 different proteins that were either identified or assigned to families. Estimation of relative protein abundances revealed that venom is dominated by three-finger toxins (77.5%; including 24.3% cytotoxins and 53.2% neurotoxins) and phospholipases A2 (13.5%). It also contains lower proportions of components belonging to nerve growth factor, ohanin/ vespryn, cysteine-rich secretory protein, C-type lectin/lectin-like, nucleotidase, phosphodiesterase, metalloproteinase, L-amino acid oxidase, cobra venom factor, and cytidyltransferase protein families. Small amounts of three nucleosides were also evidenced: adenosine, guanosine, and inosine. The most relevant lethal components, categorized by means of a ‘toxicity score’, were a-neurotoxins, followed by cytotoxins/cardiotoxins. IgGs isolated from a person who had repeatedly self-immunized with a variety of snake venoms were immunoprofiled by ELISA against all venom fractions. Stronger responses against larger toxins, but lower against the most critical a-neurotoxins were obtained. As expected, no neutralization potential against N. kaouthia venom was therefore detected. Combined, our results display a high level of venom complexity, unveil the most relevant toxins to be neutralized, and provide prospects of discovering human IgGs with toxin neutralizing abilities through use of phage display screening. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/74567 |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S004101011500063X https://hdl.handle.net/10669/74567 |
| Palabra clave: | Naja kaouthia Monocled cobra Toxicity Human IgG response Immunity Snake venom Proteomics |