Differential volatile organic compound expression in the interaction of Daldinia eschscholtzii and Mycena citricolor
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| Autores: | , , , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 2023 |
| Descripción: | Fungi exhibit a wide range of ecological guilds, but those that live within the inner tissues of plants (a.k.a. endophytes) are particularly relevant due to the benefits they sometimes provide to their hosts, such as herbivory deterrence, disease protection, and growth promotion. Recently, endophytes have gained interest as potential biocontrol agents against crop pathogens, for example, coffee plants (Coffea arabica). Published results from research done in our laboratory showed that endophytic fungi isolated from wild Rubiaceae plants were effective in reducing the effects of the American Leaf Spot of Coffee (Mycena citricolor). One of these isolates (GU11N) from the plant Randia grandifolia was identified as Daldinia eschscholtzii (Xylariales). Its antagonism mechanisms, effects, and chemistry against M. citricolor were investigated through the analysis of its volatile profile alone and in the presence of the pathogen in contactless and dual culture assays. The experimental design involved direct sampling of agar plugs in vials for headspace (HS) and headspacesolid- phase-microextraction (HS-SPME) Gas Chromatography Mass Spectrometry (GC-MS) analysis. Additionally, we used UHPLC-HRMS/MS to identify non-volatile compounds from organic extracts of the mycelia involved in the interaction. Results showed that more volatile compounds were identified using HS-SPME (39 components) than with the HS technique (13 components), sharing only 12 compounds. Statistical tests suggest that D. eschscholtzii inhibited the growth of M. citricolor through the release of VOCs containing a combination of 1,8- dimethoxynapththalene and terpene compounds affecting the M. citricolor pseudopilei. The damaging effects of 1,8-dimethoxynaphthalene were corroborated in an in vitro test against M. citricolor pseudopilei; SEM photographs confirmed the structural damage. After analyzing the UHPLC-HRMS/MS data, a predominance of fatty acid derivatives was found among the putatively identified compounds, however, a considerable proportion of features (37.3%) remained unannotated. In conclusion, our study suggests that D. eschscholtzii has a potential as a biocontrol agent against M. citricolor and that 1,8-dimethoxynaphthalene contributes to the observed damage to the pathogen’s reproductive structures. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/89926 |
| Acceso en línea: | https://hdl.handle.net/10669/89926 |
| Palabra clave: | FUNGI COFFE PLANTS PATHOLOGY ORGANIC COMPOUNDS |