Acute toxicity of frog-skin-homologous synthetic alkaloids and frog-skin extracts: miniaturized test to evaluate toxicity with Daphnia magna
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| 著者: | , , , , , , , , |
|---|---|
| フォーマット: | artículo original |
| 出版日付: | 2025 |
| その他の書誌記述: | The determination of toxicity in samples from natural extracts or products of organic synthesis represents a challenge due to the low available volume of these kinds of matrices. A miniaturized immobilization acute test in Daphnia magna was implemented at microplate-scale to determine the relative toxicity in 13 synthetic alkaloids, analogous to those found in the skin of frogs, and skin-extracts from 18 individuals of the frog Oophaga vicentei. The test revealed moderate or moderate to low toxicity towards the daphnids for all tested synthetic alkaloids after a 48-h exposure. The correlation of relative toxicity and several parameters in frog samples revealed that: (i) toxicity is not associated with frog coloration (aposematism) or their geographical location; and (ii) toxicity is dependent on the profile of alkaloid composition rather than the total alkaloid content. Results from this work provide an alternative method to assay toxicity in limited-volume samples, useful to elucidate potential aposematic-related differences in dendrobatid frogs. |
| 国: | Kérwá |
| 機関: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| 言語: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/103110 |
| オンライン･アクセス: | https://www.sciencedirect.com/science/article/pii/S0041010125003745 https://hdl.handle.net/10669/103110 https://doi.org/10.1016/j.toxicon.2025.108599 |
| キーワード: | Alkaloids Frogs Toxicity Daphnids |