Phenolic compounds as antiangiogenic CMG2 inhibitors from costa rican endophytic fungi
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| Autores: | , , , , , , |
|---|---|
| Formato: | artículo original |
| Fecha de Publicación: | 2012 |
| Descripción: | Targeting and inhibiting CMG2 (Capillary Morphogenesis Gene protein 2) represents a new strategy for therapeutic agents for cancer and retinal diseases due to CMG2’s role in blood vessel growth (angiogenesis). A high throughput FRET (Förster Resonance Energy Transfer) assay was developed for the identification of CMG2 inhibitors as anti-angiogenetic agents. Bioassay-guided separation led to the isolation and identification of two new compounds (1 and 2) from CR252M, an endophytic fungus Coccomyces proteae collected from a Costa Rican rainforest, and one known compound (3) from CR1207B (Aurapex penicillata). Secondary in vitro assays indicated anti-angiogenic activity. Compound 3 inhibited the endothelial cell migration at 52 μM, but did not show any endothelial cell antiproliferative effect at 156 μM. The structure of the two new compounds, A (1) and B (2), were elucidated on the basis of extensive spectroscopic analysis, including 1D and 2D NMR experiments. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/87970 |
| Acceso en línea: | https://www.sciencedirect.com/science/article/pii/S0960894X12009602?via%3Dihub https://hdl.handle.net/10669/87970 |
| Palabra clave: | MUSHROOM Coccomyces proteae Aurapex penicillata CMG2 Phenolic COSTA RICA |