Characterization of an R-Plasmid Associated with Ampicillin Resistance in Shigella dysenteriae Type 1 Isolated from Epidemics
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| Autores: | , , , , |
|---|---|
| Formato: | artículo original |
| Fecha de Publicación: | 1977 |
| Descripción: | Ampicillin-resistant strains of Shigella dysenteriae type 1 isolated in epidemics in Mexico, Central America, and Bangla Desh were examined for the presence of plasmid deoxyribonucleic acid (DNA) by gel electrophoresis. All strains contained a heterogeneous population of plasmids. Transfer experiments to Escherichia coli K-12 indicated that the ampicillin resistance determinant (Apr) was located on a 5.5-megadalton (Mdal) plasmid identical in all Shiga strains examined, as judged by DNA hybridization and by its molecular properties. This 5.5-Mdal plasmid contained the ampicillin transposon (TnA) sequences. There was not a high degree of homology between the Shiga Apr plasmid DNA and DNA obtained from Apr Salmonella typhi strains isolated from typhoid epidemics in Mexico, previous to the dysentery outbreaks. Although low, the degree of reassociation observed indicated that probably part of the TnA sequence was present in S. typhi DNA. The DNA hybridization experiments showed, in addition, that there was a high degree of homology among Apr plasmids isolated from different enterobacteria, and this identity was confirmed by restriction endonuclease activity. These results together with their similarities in molecular and replicative properties indicate that the Apr plasmids, as was suggested for the Smr Sur plasmids, possibly evolved once and then epidemiologically spread in the Enterobacteriaceae. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/75982 |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC352019/ https://hdl.handle.net/10669/75982 |
| Palabra clave: | Shigella dysenteriae Epidemics R-Plasmid 616.014 Bacterias |