Swollenin pre-conditioning: optimization studies and application aiming at D-lactic acid production from sugarcane bagasse
Guardado en:
| Autores: | , , , |
|---|---|
| Formato: | artículo original |
| Fecha de Publicación: | 2018 |
| Descripción: | The enzymatic hydrolysis still stands as a challenge regarding the use of renewable feedstocks, as well as of lignocellulosic materials. The present work reports optimization of swollenin addition to pretreated sugarcane bagasse, in a step prior to the enzymatic hydrolysis, named pre-conditioning. Also, it describes further fermentation of the hydrolyzed material to D-lactic acid. Trichoderma harzianum enzymatic cocktail was produced from pretreated sugarcane bagasse, while swollenin was produced using Aspergillus niger as a host for recombinant protein expression. For pre-conditioning optimization, a Central Composite Rotatable Design was applied and validated. The optimized conditions were used to produce the hydrolysate that was fermented to D-lactic acid by Lactobacillus coryniformis subsp. torquens. It could be observed that pre-conditioning effectively leads to improvements in glucose release: 62% of hydrolysis efficiency. Besides, it was possible to use the produced hydrolysate to obtain D-lactic acid, with a yield of 1.34 g/g and a productivity of 1.59 g/L h-1 . |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/89592 |
| Acceso en línea: | https://www.tandfonline.com/doi/abs/10.1080/00986445.2018.1477762?journalCode=gcec20 https://hdl.handle.net/10669/89592 |
| Palabra clave: | Enzymatic hydrolysis Lactic acid Lignocellulosic biomass Pre-conditioning Swollenin Sugarcane bagasse |