Unusual Microbial Xylanases from Insect Guts

 

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Detalles Bibliográficos
Autores: Brennan, YaLi, Callen, Walter N., Christoffersen, Leif, Dupree, Paul, Goubet, Florence, Healey, Shaun, Hernández Alfaro, Myriam, Keller, Martin, Li, Ke, Palackal, Nisha, Sittenfeld Appel, Ana, Tamayo Castillo, Giselle, Wells, Steve, Hazlewood, Geoffrey P., Mathur, Eric J., Short, Jay M., Robertson, Dan E., Steer, Brian A.
Formato: artículo original
Fecha de Publicación:2004
Descripción:Recombinant DNA technologies enable the direct isolation and expression of novel genes from biotopes containing complex consortia of uncultured microorganisms. In this study, genomic libraries were constructed from microbial DNA isolated from insect intestinal tracts from the orders Isoptera (termites) and Lepidoptera (moths). Using a targeted functional assay, these environmental DNA libraries were screened for genes that encode proteins with xylanase activity. Several novel xylanase enzymes with unusual primary sequences and novel domains of unknown function were discovered. Phylogenetic analysis demonstrated remarkable distance between the sequences of these enzymes and other known xylanases. Biochemical analysis confirmed that these enzymes are true xylanases, which catalyze the hydrolysis of a variety of substituted β-1,4-linked xylose oligomeric and polymeric substrates and produce unique hydrolysis products. From detailed polyacrylamide carbohydrate electrophoresis analysis of substrate cleavage patterns, the xylan polymer binding sites of these enzymes are proposed.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
Lenguaje:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/87893
Acceso en línea:https://journals.asm.org/doi/10.1128/AEM.70.6.3609-3617.2004
https://hdl.handle.net/10669/87893
Palabra clave:DNA technologies
Phylogenetic
INSECTS
BIOLOGÍA