MmTX1 and MmTX2 from coral snake venom potently modulate GABAA receptor activity
محفوظ في:
| المؤلفون: | , , , , , , , , |
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| التنسيق: | artículo original |
| تاريخ النشر: | 2015 |
| الوصف: | GABAA receptors shape synaptic transmission by modulating Cl− conductance across the cell membrane. Remarkably, animal toxins that specifically target GABAA receptors have not been identified. Here, we report the discovery of micrurotoxin1 (MmTX1) and MmTX2, two toxins present in Costa Rican coral snake venom that tightly bind to GABAA receptors at subnanomolar concentrations. Studies with recombinant and synthetic toxin variants on hippocampal neurons and cells expressing common receptor compositions suggest that MmTX1 and MmTX2 allosterically increase GABAA receptor susceptibility to agonist, thereby potentiating receptor opening as well as desensitization, possibly by interacting with the α+/β− interface. Moreover, hippocampal neuron excitability measurements reveal toxin-induced transitory network inhibition, followed by an increase in spontaneous activity. In concert, toxin injections into mouse brain result in reduced basal activity between intense seizures. Altogether, we characterized two animal toxins that enhance GABAA receptor sensitivity to agonist, thereby establishing a previously unidentified class of tools to study this receptor family. |
| البلد: | Kérwá |
| المؤسسة: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/30341 |
| الوصول للمادة أونلاين: | http://www.pnas.org/content/112/8/E891 https://hdl.handle.net/10669/30341 |
| كلمة مفتاحية: | Coral snake toxin MmTX1 MmTX2 GABA(A) receptor Hippocampal neurons |