Low-cost internal controls for detection of Giardia cysts in water samples

 

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Detalhes bibliográficos
Autores: Solano Barquero, Melissa, Morales Mora, Eric, Chacón Jiménez, Luz María, Cordero Jara, Erick Arnulfo, Reyes Lizano, Liliana, Barrantes Jiménez, Kenia, Achí Araya, María Rosario
Formato: artículo original
Fecha de Publicación:2019
Descrição:Giardia cysts stained with hot carbolfuchsin were used as internal controls in a concentration method for surface water samples. The morphological integrity of stained cysts and the stain's stability and intensity were tested with each of the chemical reagents used in the aluminum sulfate flocculation method. No alterations in morphology or color were noted. The stained cyst preparation has a low cost, high stability, and suitability for both light and immunofluorescent microscopy, making it affordable to researchers in low- and middle-income countries.
País:Kérwá
Recursos:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/80325
Acesso em linha:https://www.sciencedirect.com/science/article/pii/S1383576918302812?via%3Dihub
https://hdl.handle.net/10669/80325
https://doi.org/10.1016/j.parint.2019.04.012
Palavra-chave:Giardia
Internal control
Flocculation
Water