Development of an instrument-free and low-cost ELISA dot-blot test to detect antibodies against SARS-CoV-2
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Autores: | , , , , |
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Formato: | artículo original |
Fecha de Publicación: | 2023 |
Descripción: | Most laboratory tests to detect the presence of anti-SARS-CoV-2 antibodies use ELISA or CLIA; however, equipment for these immunoassays is unavailable in many areas of low- and middle-income countries. Rapid lateral flow immunoassay (LFIA) tests are a low-cost, equipment-free option, but their high price may make them less suitable for conducting seroprevalence surveys. Here, we describe a simple dual antigen ELISA dot-blot test to detect anti-SARS-CoV-2 IgG antibodies with high sensitivity (94 to 98%) and specificity (92 to 100%), compared to commercially available ELISA and CLIA options. Additionally, this ELISA dot-blot test can be completed in one hour using minimal laboratory equipment. Importantly, this immunoassay is significantly more affordable than most LFIA tests available on the global market. The dot-blot strips may be stored for up to 7 days under freezing conditions. This ELISA dot-blot test is a cost-effective option for conducting seroprevalence screenings in areas lacking ELISA or CLIA facilities, compared to LFIA tests. |
País: | Kérwá |
Institución: | Universidad de Costa Rica |
Repositorio: | Kérwá |
Lenguaje: | Inglés |
OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/89858 |
Acceso en línea: | https://hdl.handle.net/10669/89858 |
Palabra clave: | SARS-CoV-2 COVID-19 antibodies seroprevalence ELISA dot-blot IMMUNOLOGY |