A DNA pooling based system to detect Escherichia coli virulence factors in fecal and wastewater samples

 

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Detalles Bibliográficos
Autores: Cortés Bratti, Ximena, Chacón Jiménez, Luz María, Taylor Castillo, Mayra Lizeth, Valiente Álvarez, Carmen Isabel, Alvarado Pérez, Irene
Formato: artículo original
Fecha de Publicación:2012
Descripción:The availability of a useful tool for simple and timely detection of the most important virulent varieties of Escherichia coli is indispensable. To this end, bacterial DNA pools which had previously been categorized were obtained from isolated colonies as well as selected in terms of utilized phenotype; the pools were assessed by two PCR Multiplex for the detection of virulent E. coli eaeA, bfpA, stx1, stx2, ipaH, ST, LT, and aatA genes, with the 16S gene used as DNA control. The system was validated with 66 fecal samples and 44 wastewater samples. At least one positive isolate was detected by a virulent gene among the 20 that were screened. The analysis of fecal samples from children younger than 6 years of age detected frequencies of 25% LT positive strains, 8.3% eae, 8.3% bfpA, 16.7% ipaH, as well as 12.5 % aatA and ST. On the other hand, wastewater samples revealed frequencies of 25.7% eaeA positive, 30.3% stx1, 15.1% LT and 19.7% aatA. This study is an initial step toward carrying out epidemiological field research that will reveal the presence of these bacterial varieties.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/76140
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3769050/
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400012&lng=en&nrm=iso&tlng=en
https://hdl.handle.net/10669/76140
Palabra clave:Virulence genes
Wastewater
Fecal
Screening system
Escherichia coli
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