Novel three-finger toxins from Micrurus dumerilii and Micrurus mipartitus coral snake venoms: phylogenetic relationships and characterization of Clarkitoxin-I-Mdum

 

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Detalles Bibliográficos
Autores: Rey Suárez, Paola, Saldarriaga Córdoba, Mónica María, Torres Lamus, Uday Daniel, Marin Villa, Marcel, Lomonte, Bruno, Núñez Rangel, Vitelbina
Formato: artículo original
Fecha de Publicación:2019
Descripción:Micrurus mipartitus and M. dumerilii are the most medically important coral snakes in Colombia. Proteomic characterization of their venoms has previously shown that proteins of the three-finger toxin (3FTx) family are abundant components, especially in M. mipartitus (61%) and to a lesser extent in M. dumerilii (28%). In order to increase knowledge on these toxins, in this work a major 3FTx of M. dumerilii venom (8% of the venom proteins), named Clarkitoxin-I-Mdum, was isolated and characterized. Its amino acid sequence comprises 66 residues, with an isotope-averaged molecular mass of 7537 ± 2 Da and a theoretical pI of 9.36, presenting the conserved pattern of eight cysteines that classifies it as a short-chain (type I) 3FTx. Clarkitoxin-I-Mdum was not lethal to mice by intravenous or intracerebroventricular route and was not cytolytic to myogenic cells in vitro. On the other hand, five coding sequences for 3FTxs were obtained from the venom gland of M. mipartitus. These novel toxin sequences were named Mm3FTx-01 to Mm3FTx-05, all of them also presenting the eight conserved cysteines of short-chain 3FTxs. Phylogenetic analysis revealed high variability of 3FTxs from Micrurus, and ELISA using antibodies raised to the major 3FTxs from M. mipartitus and M. dumerilii confirmed their immunochemical divergence. These results highlight the relevance of performing further studies aiming at a deeper understanding of the functional and antigenic relationships among specific Micrurus toxins, with important implications for the production of antivenoms.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:https://www.kerwa.ucr.ac.cr:10669/80384
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S0041010119304593?via%3Dihub
https://hdl.handle.net/10669/80384
Access Level:acceso abierto
Palabra clave:Micrurus
α-neurotoxin
Coral snake
Micrurus dumerilii
Micrurus mipartitus
Three-finger toxins
Snake venom