First Report of the Isolation and Molecular Characterization of Rickettsia amblyommii and Rickettsia felis in Central America


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Bibliographic Details
Authors: Hun Opfer, Ruchilia Laya, Troyo Rodríguez, Adriana, Taylor Castillo, Mayra Lizeth, Barbieri, Amália R. M., Labruna, Marcelo B.
Format: artículo original
Publication Date:2011
Description:During 2010, 15 adult ticks, identified as Amblyomma cajennense, were collected from horses in Cahuita and Turrialba districts, whereas 7 fleas, identified as Ctenocephalides felis, were collected from a dog in San Jose city, Costa Rica. In the laboratory, three A. cajennense specimens, two from Cahuita and one from Turrialba, were individually processed for rickettsial isolation in cell culture, as was a pool of seven fleas. Rickettsiae were successfully isolated and established in Vero cell culture from the three ticks and from a pool of seven fleas in C6/36 cell culture. The three tick isolates were genotypically identified as Rickettsia amblyommii, and the flea isolate was identified as Rickettsia felis through DNA sequencing of portions of the rickettsial genes gltA, ompA, and ompB of each isolate. In addition, other seven ticks were shown to contain rickettsial DNA. Polymerase chain reaction products of at least two of these ticks were sequenced and also showed to correspond to R. amblyommii. Overall, 66.7% (10/15) of the A. cajennense adult ticks were found to be infected with rickettsiae. This is the first report of a successful isolation in cell culture of R. amblyommii and R. felis from Central America.
Institution:Universidad de Costa Rica
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Access Level:acceso abierto
Keyword:Rickettsia felis
Rickettsia amblyommii
Ctenocephalides felis
Costa Rica
Amblyomma cajennense
636.089 medicina veterinaria