First isolation and genomic insights of Bartonella henselae from domestic cats and dogs in Costa Rica
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| Συγγραφείς: | , , , , , , , |
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| Μορφή: | artículo |
| Ημερομηνία έκδοσης: | 2026 |
| Περιγραφή: | Bartonella spp. are vector-borne zoonotic pathogens responsible for cat-scratch disease and several other human disease manifestations. Previous studies in Costa Rica have reported Bartonella DNA in ectoparasites and bats, but data from companion animals remain limited. Here, we investigated the occurrence of Bartonella spp. in domestic cats and dogs and characterized circulating strains using molecular, culture-based, and genomic approaches. Blood samples were collected from 152 domestic cats and 188 domestic dogs from different Costa Rican regions. Conventional PCR assays targeting citrate synthase (gltA) and the beta-subunit of bacterial RNA polymerase (rpoB) detected Bartonella spp. DNA in 44% of cats and 34% of dogs. Partial gltA sequencing identified B. henselae, B. rochalimae and B. clarridgeiae. Four B. henselae isolates recovered from feline blood were subjected to whole-genome sequencing. Comparative pan-genome and multilocus sequence typing (MLST) analyses identified ST1, ST5 sequence types circulating in Costa Rica. These findings provide the first genomic characterization of Bartonella spp. from companion animals in Costa Rica and expand current knowledge of the genetic diversity and epidemiology of Bartonella in Central America. |
| Χώρα: | Kérwá |
| Ίδρυμα: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Γλώσσα: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104804 |
| Διαθέσιμο Online: | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2026.1870135/abstract https://hdl.handle.net/10669/104804 |
| Λέξη-Κλειδί : | Bartonella henselae Bartonella isolation vector-borne disease zoonosis companion animals pan-genome analysis Costa Rica |