Resurrexit, sicut dixit, alleluia. Snake venomics from a 26-years old polyacrylamide focusing gel

 

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Detalles Bibliográficos
Autores: Righetti, Pier Giorgio, Lomonte, Bruno, Calvete Chornet, Juan José
Formato: artículo original
Fecha de Publicación:2012
Descripción:A 26-year-old dried polyacrylamide gel, cast in presence of an immobilized pH gradient and containing focused proteins from the venoms of a northern black-tailed rattlesnake (Crotalus molossus molossus), and of a western diamondback rattlesnake (Crotalus atrox) has been screened in order to see the feasibility of extracting the proteins, analyzing thembymass spectrometry (MS) and assessing their integrity.Nine gel bandswere excised along the pH 3–10 gradient and the gel segments reswollen inwarmacetonitrile. Upon digestion andMSanalysis, all the bands could be identified and attributed to the respective venoms of the two rattlesnake species. Although a few peptides exhibited modified amino acids, the proteins were found to be well preserved even upon such a long storage at room temperature. The present data suggest the feasibility of identifying proteins from very old samples trapped in polyacrylamide gels, and analyzed in a pre-mass spectrometry era, thus of uncertain identity.
País:Kérwá
Institución:Universidad de Costa Rica
Repositorio:Kérwá
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/74491
Acceso en línea:https://www.sciencedirect.com/science/article/pii/S1874391911004751
https://hdl.handle.net/10669/74491
Palabra clave:Snake venomics
Dried polyacrylamide gels
Protein recovery
Mass spectrometry
Immobilized pH gradients
Venómica
Venomics
Proteómica
Proteomics