Bone tissue processing and sterilization for therapeutic use: preclinical experiments from a technological university

 

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Nhiều tác giả: Centeno-cerdas, Carolina, Somarribas-Brenes, Fabián, Vargas-Segura, Walter, Jarquín-Cordero, Monstserrat, Ulloa-Fernández, Andrea, Calvo-Castro, Laura
Định dạng: artículo original
Trạng thái:Versión publicada
Ngày xuất bản:2024
Miêu tả:Gamma irradiation is among the most widely used methods of bone sterilization. The usual standard dose for achieving sterilization is 25 kGy. Although there are international standards for the main procedures, the general recommendation is to validate them at the local level, so that they are feasible with the personnel and technical capacities of the country. In this study, porcine bone tissue samples were treated by chemical disinfection and by sterilization with gamma irradiation, reducing the microbial load to non-detectable levels, without significantly affecting the desirable properties of the tissue matrix, which was verified by protein characterization of collagen and bone morphogenetic proteins (BMPs) and by scanning electron microscopy. This research demonstrates the ability of Costa Rica Institute of Technology (ITCR) to contribute to the training of professionals and to provide access to specialized equipment, which allows the strengthening of tissue banks in the country, and thereby contributing to the development of new technological strategies for human health.
Quốc gia:Portal de Revistas TEC
Tổ chức giáo dục:Instituto Tecnológico de Costa Rica
Repositorio:Portal de Revistas TEC
Ngôn ngữ:Español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/7625
Truy cập trực tuyến:https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/7625
Từ khóa:Bone
gamma irradiation
tissue banking
hydrogen peroxide
Hueso
irradiación gamma
banco de tejidos
peróxido de hidrógeno