Bone tissue processing and sterilization for therapeutic use: preclinical experiments from a technological university

 

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Συγγραφείς: Centeno-cerdas, Carolina, Somarribas-Brenes, Fabián, Vargas-Segura, Walter, Jarquín-Cordero, Monstserrat, Ulloa-Fernández, Andrea, Calvo-Castro, Laura
Μορφή: artículo original
Κατάσταση:Versión publicada
Ημερομηνία έκδοσης:2024
Περιγραφή:Gamma irradiation is among the most widely used methods of bone sterilization. The usual standard dose for achieving sterilization is 25 kGy. Although there are international standards for the main procedures, the general recommendation is to validate them at the local level, so that they are feasible with the personnel and technical capacities of the country. In this study, porcine bone tissue samples were treated by chemical disinfection and by sterilization with gamma irradiation, reducing the microbial load to non-detectable levels, without significantly affecting the desirable properties of the tissue matrix, which was verified by protein characterization of collagen and bone morphogenetic proteins (BMPs) and by scanning electron microscopy. This research demonstrates the ability of Costa Rica Institute of Technology (ITCR) to contribute to the training of professionals and to provide access to specialized equipment, which allows the strengthening of tissue banks in the country, and thereby contributing to the development of new technological strategies for human health.
Χώρα:Portal de Revistas TEC
Ίδρυμα:Instituto Tecnológico de Costa Rica
Repositorio:Portal de Revistas TEC
Γλώσσα:Español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/7625
Διαθέσιμο Online:https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/7625
Λέξη-Κλειδί :Bone
gamma irradiation
tissue banking
hydrogen peroxide
Hueso
irradiación gamma
banco de tejidos
peróxido de hidrógeno