Use of gamma rays to produce Echeveria sp. mutants to boost the future profit of the national producer
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| Autores: | , , , |
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| 格式: | artículo original |
| 状态: | Versión publicada |
| Fecha de Publicación: | 2025 |
| 实物特征: | Radioinduced mutagenesis is a powerful tool for enhancing genetic variability in plants, enabling the induction of desired traits, absent in nature or lost through evolution and breeding. This study aimed to generate mutant lines of Echeveria sp. using gamma rays, expanding the variety of succulents available in Costa Rica. A disinfection treatment using 20 min of incubation in NaOCl resulted in 46.9 % aseptic explants. The medium containing 50 % MS salts and 6 g.L-1 of gelling agent proved to be the most effective for explant multiplication and hyperhydricity reduction. Leaves from Echeveria sp. vitroplants were exposed to gamma radiation doses of 0, 20, 40, 60, 80 and 100 Gy. The LD50 for vitroplant leaves was determined to be 23.16 Gy. Irradiated explants were subcultured in multiplication medium to promote sprouting and, after six subcultures, successfully acclimatized in greenhouse conditions. The established irradiation and micropropagation protocols proved to be effective for mutation induction in Echeveria sp., offering an innovative resource for developing new ornamental varieties and supporting their adoption by succulent producers. |
| País: | Portal de Revistas TEC |
| 机构: | Instituto Tecnológico de Costa Rica |
| Repositorio: | Portal de Revistas TEC |
| 语言: | Español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/7593 |
| 在线阅读: | https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/7593 |
| Palabra clave: | Biotechnology Atomic Energy mutation radiation plant genetic resources Biotecnología energía atómica mutación radiación recursos vegetales |