Optimization of somatic embryogenesis in Coffea arabica: evaluation of leaf explant orientation and origin
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| Autoři: | , , |
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| Médium: | artículo original |
| Stav: | Versión publicada |
| Datum vydání: | 2024 |
| Popis: | Coffee (C. arabica) farming in Costa Rica is facing several challenges due to climatic changes and market fluctuations. The search for higher quality and more resistant varieties has led to the research of new alternative propagation methods, such as somatic embryogenesis (SE), which allows the generation of new subspecies under controlled laboratory conditions. Direct propagation, without callus, has a significant influence on explant success depending on leaf orientation and the origin of the sample. To prove this, this work evaluates the effect of the adaxial and abaxial orientation of C. arabica leaves obtained from distal, proximal and medial points of vitroplants for the induction of direct somatic embryogenesis. Leaf sections were placed into supplemented Murashige and Skoog (MS) culture medium. Multivariate analysis was performed to know which combination of treatments led to a better response to SE, and it is shown using a heat map. About 36% regeneration callus was produced by introducing explants in adaxial form with decreased oxidation. Furthermore, distal and medial parts exhibited higher regenerative capacity. Adaxial orientation of C. arabica leaves promoted somatic embryo induction possibly by enhancing gas exchange due to hypostomatic leaf feature. By optimizing this protocol, the plant introduction process from leaves may beimproved with a higher expected success rate. |
| Země: | Portal de Revistas TEC |
| Instituce: | Instituto Tecnológico de Costa Rica |
| Repositorio: | Portal de Revistas TEC |
| Jazyk: | Español |
| OAI Identifier: | oai:ojs.pkp.sfu.ca:article/7620 |
| On-line přístup: | https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/7620 |
| Klíčové slovo: | In vitro culture callus coffee leaf vitroplant Cultivo in vitro callo café hoja vitroplanta |