In vitro assays to quantify the biological activity of cytokines

 

Αποθηκεύτηκε σε:
Λεπτομέρειες βιβλιογραφικής εγγραφής
Συγγραφείς: Carrodeguas-González, Ayerim, Zúñiga-Orozco, Andres, Ortíz-Cruz, Maria Victoria
Μορφή: artículo original
Κατάσταση:Versión publicada
Ημερομηνία έκδοσης:2022
Περιγραφή:Cytokines are low molecular weight molecules that play a fundamental role in the inflammatory and immune response and in numerous biological and cellular processes. They are the active ingredient in many drugs, produced to treat different diseases, many of these related to the immune system. These drugs, before being marketed, must meet a series of requirements, among which is the quantification of biological activity. The biological activity of cytokines is measured through in vivo and in vitro biological tests, the latter being the most widely used due to their greater speed and versatility. In vitro tests are carried out in places with all the necessary equipment and personnel with sufficient experience to carry them out. There are many factors that can influence the quality of these analytical methods, which must be kept under strict control for approval. For these reasons, the objective of this review is to gather the theoretical bases for conducting in vitro assays to quantify the biological activity of cytokines, as well as the factors that influence their quality.
Χώρα:Portal de Revistas TEC
Ίδρυμα:Instituto Tecnológico de Costa Rica
Repositorio:Portal de Revistas TEC
Γλώσσα:Español
OAI Identifier:oai:ojs.pkp.sfu.ca:article/5638
Διαθέσιμο Online:https://revistas.tec.ac.cr/index.php/tec_marcha/article/view/5638
Λέξη-Κλειδί :bioassays
cell lines
contamination
biological potency
drugs
parallelism
ensayos biológicos
líneas celulares
contaminación
potencia biológica
fármacos
paralelismo