A Protocol to Perform Systemic Lipopolysacharide (LPS) Challenge in Rats
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| Autoři: | , , |
|---|---|
| Médium: | artículo original |
| Stav: | Versión publicada |
| Datum vydání: | 2019 |
| Popis: | Lipopolysaccharide (LPS) is a component of the outer membrane of Gram-negative bacteria. In animals, intraperitoneal administration of LPS, stimulates innate immunity and the production of pro-inflammatory cytokines. LPS provides an inflammatory stimulus that activates the neuroimmune and neuroendocrine systems resulting in a set of responses termed sickness behavior. The purpose of this protocol is to describe step-by-step the preparation and procedure of application of intraperitoneal injection of LPS in rats, as a guide for those researchers that want to use this assay to mount an inflammatory response. LPS intraperitoneal challenge in rats has been widely used to evaluate anti-inflammatory reagents and to address basic scientific questions. |
| Země: | Portal de Revistas UCR |
| Instituce: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Jazyk: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/35510 |
| On-line přístup: | https://revistas.ucr.ac.cr/index.php/Odontos/article/view/35510 |
| Klíčové slovo: | Lipopolysaccharide; LPS challenge; LPS stimulation; Endotoxin; Inflammation |