Immuno-gold replica technique for ultrastructural localization of antigens
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| Авторы: | , , |
|---|---|
| Формат: | artículo original |
| Статус: | Versión publicada |
| Дата публикации: | 1994 |
| Описание: | We developed a simple method for immunolabelling viral glycoproteins on surface of infected ceil monolayers. Cells 9 hours post-infected with Sendai virus were fixed and treated with polyclonal antibody anti HN glycoprotein (Rabbit IgG), labelled with goat immunoglobulin anti rabbit IgG conjugated with colloidal gold particles. Then, the cells were shadowed with platinum-carbon, covered with nitrocellulose glue, and digested with domestic bleach, washed, and replica fragments were caught with uncovered grid. |
| Страна: | Portal de Revistas UCR |
| Институт: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Язык: | Inglés |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/28717 |
| Online-ссылка: | https://revistas.ucr.ac.cr/index.php/rbt/article/view/28717 |
| Access Level: | acceso abierto |
| Ключевое слово: | Sendai virus immunolabelling immunoelectronmicroscopy replica ultrastructure |