Isolation and molecular identification of fungi in bananas with and without leaf spots
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| Autori: | , , , , |
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| Natura: | artículo original |
| Status: | Versión publicada |
| Data di pubblicazione: | 2026 |
| Descrizione: | Introduction. The foliar structure of banana plants is a determining factor in the yield and development of the crop. The microbial composition in foliar spots is relevant for understanding the emergence and spread of diseases. Objective. To perform the isolation and molecular identification of fungi in banana plants with and without leaf spots. Materials and Methods. In 2019, a non-experimental research study was conducted with a quantitative approach and descriptive level, using banana leaves from Musa acuminate (cv.IC2) with spots (HCM) and without spots (HSM), collected from a conventionally managed agricultural plot in northern Peru. Leaf fragments of 5 x 5 mm were cultivated on microbiological media, and fungal strains were isolated based on their macrostructures. DNA was extracted from the strains, and conventional PCR was performed targeting the ITS region of fungi (700 bp). The PCR products were then sequenced using the Sanger method in double strands. Taxonomic assignments at the species level were made using the BLAST tool, based on homology comparison with sequences from GenBank. Results. A total of eleven fungal species were identified in HCM and eight in HSM, including both phytopathogenic and non-phytopathogenic species. The predominant species in HCM were Fusarium spp., Cladosporium cladosporioides, and Lasiodiplodia theobromae. Additionally, it was observed that HCM shared three genera with HSM: Nigrospora, Cladosporium, and Fusarium. Conclusion. Fungal isolation and molecular identification were performed on bananas with and without leaf spots, and phytopathogenic and beneficial species were found. |
| Stato: | Portal de Revistas UCR |
| Istituzione: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Lingua: | Español |
| OAI Identifier: | oai:portal.revistas.ucr.ac.cr:article/1678 |
| Accesso online: | https://revistas.ucr.ac.cr/index.php/ragromeso/article/view/1678 |
| Keyword: | Musa acuminata phytopathology microbiote ADN PCR fitopatología microbiota |