Gene expression in Longissimus dorsi and liver in two stages of growth in pigs
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| Autores: | , , , |
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| Formato: | texto |
| Estado: | Versión publicada |
| Fecha de Publicación: | 2024 |
| Descripción: | Introduction. It is said that genetic expression varies in relation to the physiological stage of the pig and the nutritional source, it is different in muscle and liver. Objective. Identify the genes that present differential genetic expression, through transcriptome analysis in Longissimus dorsi and liver, during growth and final fattening, differentiating them into two stages of pig fattening, growth group (55 ± 1.05 kg) and final group (101 ± 7.8kg). Materials and methods. 12 total samples were considered, three from Longissimus dorsi muscle and three from liver per stage for RNA extraction and sequencing. With the DESeq2 method, the gene expression of the Log2FC was obtained differentially for the Growth Group vs final fattening of Longissimus dorsi and Liver and the biological function of the DEG genes was identified. Results. The largest number of genes with DEG were identified in the liver and on chromosome 6 in Longissimus dorsi and liver. In Longissimus dorsi from the growth group with high expression, the genes FUT1, SESN2 and FGF21 associated with growth and with low expression, NR4A3, PDK4, PER1 and PTPRO involved in immune system processes and circadian rhythm were identified. In the liver of the Growth Group, with high expression, the IHH and MYL7 genes were identified, and with low expression, the genes MFSD2A, LIPG, THBS1, TGFB2, LTF and APOA4 were identified as those most involved in biological processes. Conclusions. In Longissimus dorsi from the growth group the genes were related to growth and in final fattening with immunity, growth and meat quality. In the liver of the growth group the genes were related to growth and in the final fattening group with immunity, growth, nutrient and lipid metabolism, lipoproteins and detoxification. |
| País: | Portal de Revistas UCR |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Lenguaje: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/57540 |
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/agromeso/article/view/57540 |
| Palabra clave: | cromosome transcriptome DESeq2 method cromosoma transcriptoma método |