Genetic diversity of passion fruits in Guatemala revealed by AFLP markers.

 

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Autores: Ponciano-Samayoa, Karla Melina, Lacán de León, Juan Pedro
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2012
Descripción:Genetic diversity of passion fruits in Guatemala revealed by AFLP markers. The objectives of this study were to characterize with AFLP nine genotypes of passion fruit collected in Guatemala, to identify clusters and to evaluate the existing genetic diversity. The study was conducted at Instituto de Ciencia y Tecnología Agrícolas (ICTA), during July 2010/May 2011. A preliminary analysis was conducted in which ten selective primer pairs were amplified and 106 polymorphisms were detected. The selective primer pair E+ACG/M+CAG, E+ACA/M+CTA, E+ACT/M+CTG and E+AAC/M+CTT, gave the most information. An average often loci per selective amplification was detected. Similarity analysis revealed no duplicated genotypes. Correspondence and clustering analysis identified two groups. The fi rst one included all accession of P. edulis f. edulis Sims and the second one all accessions of P. edulis f. fl avicarpa Degener.The genetic diversity based on Nei´s index for the collection was 0,3160. Genetic differentiation (Gst) was 0,2542 for theentire collection; 25.42% of the genetic diversity is expressed between groups while the 74,58% is expressed within groups. Those results give evidence of the evolutionary proximity of the purple and yellow types of P. edulis Sims.The genetic flow was high (Nm=1,4670) as expected in anallogamous species in which genetic exchange is facilitated.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/2136
Acceso en línea:https://revistas.ucr.ac.cr/index.php/agromeso/article/view/2136
Access Level:acceso abierto
Palabra clave:Passiflora edulis Sims
similarity
genetic differentiation
clusters
genetic flow.
similaridad
diferenciación genética
conglomerados
flujo genético.