Genetic resistance of tomato hybrids [Solanum lycopersicum L. (Mill.)] to tomato spotted wilt virus (TSWV)
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| Autores: | , , , , , |
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| Formato: | artículo original |
| Estado: | Versión publicada |
| Fecha de Publicación: | 2013 |
| Descripción: | This research was conducted at the PROINPA Foundation’s greenhouse and laboratory in Cochabamba, Bolivia in 2012. Its objective was to evaluate the resistance and susceptibility to Tomato spotted wilt virus – TSWV, Tomato cholorotic spot virus – TCSV and Groundnut ringspot virus – GRSV in 10 tomato hybrids. Phenotypic and molecular pattern (SCAR marker SW-421) evaluations were performed in order to differentiate homozygous and heterozygous resistant from susceptible plants. Results showed that molecular marker Sw421 is co-located with the TSWV-resistance Sw-5 gene. A TSWV-resistance band (R) was observed at 940 bp and showed the homozygous presence of the Sw-5 allele (Sw-5/Sw-5) in PROINPA 2 (Aguai) and PROINPA 9 (Bonita) varieties. PROINPA 1 (Andinita), PROINPA 3 (Arami), PROINPA 4 (Yara), PROINPA 5 (Pintona), PROINPA 6 (Jasuka) and PROINPA 10 (Bola Pera) varieties, showed a TSWV resistance band (H) at 900- 940 bp in the heterozygous state (Sw-5/Sw-5+). Only PROINPA 7 (Redonda), the male parent 71 LACHING 89S Sw-5 and the variety Shannon showed TSWV susceptibility gene (S) at 900 bp in the homozygous-recessive state (Sw-5+/Sw-5+). The results of the severity analysis and of DAS- ELISA were confirmed by the molecular analysis. |
| País: | Portal de Revistas UCR |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Lenguaje: | Español |
| OAI Identifier: | oai:portal.ucr.ac.cr:article/10713 |
| Acceso en línea: | https://revistas.ucr.ac.cr/index.php/agrocost/article/view/10713 |
| Palabra clave: | DAS-ELISA severity homozygous heterozygous susceptibility allele severidad homocigosis heterocigosis susceptibilidad alelo |