Genotoxic evaluation of Río Grande (Antioquia, Colombia) water using micronucleus frequency in erythrocytes of Brycon henni (Characiformes: Characidae)

 

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Autores: Zapata Restrepo, Lina María, Orozco Jiménez, Luz Yaneth, Rueda Cardona, Maribel, Echavarría, Sandra Liliana, Palacio Baena, Jaime Alberto
Formato: artículo original
Estado:Versión publicada
Fecha de Publicación:2017
Descripción:The permanent monoculture of bananas and plantains farming in the middle of Río Grande (Turbo - Antioquia) requires the application of a variety of pesticides. Inappropriate banana production practices in this region, have often led to waterbody pollution by agrochemicals from leachate and runoff processes. Currently, fish are the most common vertebrates used as bioindicators of water quality, because they are very sensitive to the presence of contaminants. Our main goal with this study was to compare the frequency of micronucleated erythrocytes (MNE) in peripheral blood specimens of fish Brycon henni, from two locations (polluted and unpolluted) in the Rio Grande. We evaluated the frequency of MNE in peripheral blood samples of fish B. henni from each location during two rainy seasons in 2010 and two dry seasons in 2011. Blood samples were collected, fixed for 24 h, and then were stained with Giemsa. Among results, we found that the median frequency of MNE was higher in the polluted site by agrochemical discharges (0.15±0.18), than in the unimpacted site (0.06±0.08). Furthermore, the frequency of MNE in B. henni during the dry season was highly significant for both locations. The results of this study indicated that the analysis of MNE in B. henni could be recommended as a suitable method for in situ detection of environmental genotoxins.
País:Portal de Revistas UCR
Institución:Universidad de Costa Rica
Repositorio:Portal de Revistas UCR
Lenguaje:Español
OAI Identifier:oai:portal.ucr.ac.cr:article/21766
Acceso en línea:https://revistas.ucr.ac.cr/index.php/rbt/article/view/21766
Access Level:acceso abierto
Palabra clave:frequency of micronucleated erythrocytes
biomarker
agrochemicals
Brycon henni
DNA damage
frecuencia de eritrocitos micronucleados
biomarcadores
agroquímicos
daño en el ADN.