Reverse-Transcription Loop-Mediated Isothermal Amplification and alternative protocols for lower cost, large-scale COVID-19 testing: lessons from an emerging economy
Gorde:
| Egileak: | , , , , |
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| Formatua: | artículo original |
| Egoera: | Versión publicada |
| Argitaratze data: | 2022 |
| Deskribapena: | Introduction: Most successful cases of COVID-19 pandemic mitigation and handling have relied on extensive reverse-transcription quantitative polymerase chain reaction (RT-qPCR). However, many emerging economies have struggled with current molecular testing demands due to economic, technical and technological constraints. Objective: To define a potential diagnostic protocol to increase testing capacity in current and post-pandemic conditions. Methods: We reviewed the literature, patents and commercial applications, for alternatives. Results: We found a good potential in saliva samples, viral inactivation and quick RNA extraction by heating; the use of an isothermal technology such as loop mediated isothermal amplification (LAMP) and naked eye test-result visualization by in-tube colorimetry or turbidity. Conclusions: Saliva samples with quick RNA extraction by heating and colorimetric LAMP are promising options for countries with economic and infrastructure limitations. |
| Herria: | Portal de Revistas UCR |
| Erakundea: | Universidad de Costa Rica |
| Repositorio: | Portal de Revistas UCR |
| Hizkuntza: | Inglés |
| OAI Identifier: | oai:archivo.portal.ucr.ac.cr:article/47407 |
| Sarrera elektronikoa: | https://archivo.revistas.ucr.ac.cr/index.php/rbt/article/view/47407 |
| Gako-hitza: | emerging economies molecular testing SARS-CoV-2 saliva loop mediated isothermal amplification economías emergentes pruebas moleculares amplificación isotérmica mediada por bucle |