Brucella abortus transits through the autophagic pathway and replicates in the endoplasmic reticulum of nonprofessional phagocytes

 

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Detalles Bibliográficos
Autores: PIZARRO CERDA, JAVIER, MERESSE, STEPHANE, PARTON, ROBERT, GISOU, VAN DER, SOLA LANDA, ALBERTO, LOPEZ GOÑI, IGNACIO, GORVEL, JEAN PIERRE, Moreno, Edgardo
Formato: artículo
Fecha de Publicación:1988
Descripción:Brucella abortus is an intracellular pathogen that replicates within a membrane-bounded compartment. In this study, we have examined the intracellular pathway of the virulent B. abortus strain 2308 (S2308) and the attenuated strain 19 (S19) in HeLa cells. At 10 min after inoculation, both bacterial strains are transiently detected in phagosomes characterized by the presence of early endosomal markers such as the early endosomal antigen 1. At ;1 h postinoculation, bacteria are located within a compartment positive for the lysosome associated membrane proteins (LAMPs) and the endoplasmic reticulum (ER) marker sec61b but negative for the mannose 6-phosphate receptors and cathepsin D. Interestingly, this compartment is also positive for the autophagosomal marker monodansylcadaverin, suggesting that S2308 and S19 are located in autophagic vacuoles. At 24 h after inoculation, attenuated S19 is degraded in lysosomes, while virulent S2308 multiplies within a LAMP- and cathepsin D-negative but sec61b- and protein disulfide isomerase-positive compartment. Furthermore, treatment of infected cells with the pore-forming toxin aerolysin from Aeromonas hydrophila causes vacuolation of the bacterial replication compartment. These results are compatible with the hypothesis that pathogenic B. abortus exploits the autophagic machinery of HeLa cells to establish an intracellular niche favorable for its replication within the ER
País:Repositorio UNA
Institución:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
Lenguaje:Inglés
OAI Identifier:oai:null:11056/22949
Acceso en línea:http://hdl.handle.net/11056/22949
https://doi.org/10.1128/IAI.66.12.5711-5724.1998
Palabra clave:BRUCELLA ABORTUS
BRUCELOSIS
BACTERIAS
BACTERIA