Studying the interaction of neutrophils and glaesserella parasuis indicates a serotype independent benefit from degradation of NETs

 

Guardado en:
Detalles Bibliográficos
Autores: Bonilla, Marta C., Lassnig, Simon, Obando Corella , Andrea, Imker, Rabea, Valentin-Weigand, Peter, von Köckritz-Blickwede, Maren, Luther, Anne-Marie, Hennig-Pauka, Isabel, de Buhr, Nicole
Formato: artículo
Fecha de Publicación:2022
Descripción:Glaesserella (G.) parasuis is one of the most important porcine pathogens causing Glaesser’s disease. Neutrophil granulocytes are the major counteracting cell type of the innate immune system, which contribute to the host defense by phagocytosis or the formation of neutrophil extracellular traps (NETs). Recently, NET-formation has been shown to facilitate the survival of bacteria from the Pasteurellaceae family. However, the interaction of NETs and G. parasuis is unclear so far. In this study, we investigated the interplay of three G. parasuis serotypes with porcine neutrophils. The production of reactive oxygen species by neutrophils after G. parasuis infection varied slightly among the serotypes but was generally low and not significantly influenced by the serotypes. Interestingly, we detected that independent of the serotype of G. parasuis, NET formation in neutrophils was induced to a small but significant extent. This phenomenon occurred despite the ability of G. parasuis to release nucleases, which can degrade NETs. Furthermore, the growth of Glaesserella was enhanced by external DNases and degraded NETs. This indicates that Glaesserella takes up degraded NET components, supplying them with nicotinamide adenine dinucleotide (NAD), as this benefit was diminished by inhibiting the 50 -nucleotidase, which metabolizes NAD. Our results indicate a serotype-independent interaction of Glaesserella with neutrophils by inducing NET-formation and benefiting from DNA degradation.
País:Repositorio UNA
Institución:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
Lenguaje:Inglés
OAI Identifier:oai:null:11056/24319
Acceso en línea:https://www.mdpi.com/journal/pathogens
http://hdl.handle.net/11056/24319
https://doi.org/10.3390/pathogens11080880
Palabra clave:BACTERIAS
PATOLOGÍA ANIMAL
ANIMAL PATOLOGY
CERDO
SWINE
NEUTROPHIL EXTRACELLULAR TRAPS (NETS)