Characterization of Anaplasma spp. infection in dogs from Costa Rica

 

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Detalles Bibliográficos
Autores: Dolz, Gaby, Bonilla, Marta C., Campos-Calderón, Liliana, Jiménez Rocha, Ana Eugenia, Romero-Zúñiga, Juan José, Alberti, Alberto, ZOBBA, Rosanna
Formato: artículo
Fecha de Publicación:2017
Descripción:A cross-sectional study combining serological and molecular techniques for detecting selected Anaplasma species was conducted between 2011 and 2012 in dogs and ticks sampled in all provinces of Costa Rica. Global Anaplasma spp. seroprevalence was 2.7% (11/408) by indirect immunofluorescence assay. The 16S rRNA PCR confirmed active A. phagocytophilum infection only in one dog (0.3%, 1/374); however, the same sample was negative to groEL PCR. Out of 122 Rhipicephalus sanguineus s.l. ticks analyzed, one (0.8%) was found positive to A. phagocytophilum 16S rRNA PCR but negative when tested by groEL PCR; this tick was collected from a seronegative and PCR negative dog. Both 16S rRNA sequences were 100% (510/510 bp) identical to A. phagocytophilum strains isolated in different countries from different hosts. The presence of A. platys was established in four dogs (1%, 4/374) by both 16SrRNA and groEL PCR. Ticks collected from the same dogs tested negative by PCR. The 16S rRNA sequences were 100% identical to the corresponding sequences of A. platys strains isolated from dogs in Croatia and Brazil, however groEL sequences showed variable similarity levels (99–100%) with different strains of A. platys isolated in Chile, Japan and Thailand, pointing out the possible presence of different variants in Central America. Collectively data indicate low prevalence of A. phagocytophilum and A. platys in dogs from Costa Rica. Furthermore, infections seem to occur without clinical signs but with some hematological changes, and seem to resolve without treatment.
País:Repositorio UNA
Institución:Universidad Nacional de Costa Rica
Repositorio:Repositorio UNA
Lenguaje:Inglés
OAI Identifier:oai:null:11056/17640
Acceso en línea:http://hdl.handle.net/11056/17640
https://doi.org/10.1016/j.vprsr.2017.02.003
Palabra clave:PCR
PERRO
ANAPLASMOSIS
ANAPLASMA PLATYS
SEROLOGY
COSTA RICA