In vitro manipulation of egg activation in the open thelycum shrimp Litopenaeus
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| Autores: | , |
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| Formato: | artículo |
| Fecha de Publicación: | 2007 |
| Descripción: | This research was designed to develop a basis for the in vitro manipulation of egg activation in Litopenaeus. The timing and sequence of events associated with egg activation of three species of Litopenaeus: L. occidentalis, L. stylirostris, and L. vannamei are described. Spawning in cold seawater (21.5 °C) induces a significant delay in cortical rod release as well as in hatching envelope formation. Magnesium concentration at 318.8 and 5.2 mg l−1 did not induce any detectable variation in egg activation. In vitro availability of unfertilized eggs was accomplished by dissecting ovaries at the time of spawning; a latency period in dry conditions from 1 to 10 min induced no detrimental effect on initiation of egg activation. In addition, an in vitro assay for evaluating interspecific and intraspecific primary binding among species of Litopenaeus is presented. © 2006 Elsevier B.V. All rights reserved. |
| País: | Repositorio UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:null:11056/24274 |
| Acceso en línea: | http://dx.doi.org/10.1016/j.aquaculture.2006.12.025 http://hdl.handle.net/11056/24274 |
| Palabra clave: | SHRIMP CRUSTÁCEOS DENDROBRANCHIATA REPRODUCCIÓN ANIMAL ANIMAL REPRODUCTION FECUNDACIÓN IN VITRO |