A Selective Ion HPLC-APCI-MS Method for the Quantification of Pentacyclic Triterpenes in an Anxiolytic Botanical Dietary Supplement for the Animal Health Market
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| Autores: | , , , , , , , , , , , , , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2019 |
| Descripción: | A new anti-anxiety dietary supplement was developed for the animal health market, by combining 2 triterpene containing botanicals, Souroubea sympetala (Marcgraviaceae) with Platanus occidentalis (Platanaceae) A validated method for a quality control of the botanical blend was achieved using HPLC-APCIMS. The method resulted in the detection and quantitative determination of betulinic acid (1), and ursolic acid (2) in P. occidentalis and 1, 2, lupeol (3), β-amyrin (4) and α-amyrin (5) in S. sympetala and the finished product ZentrolTM. Detections were at low ng on column for 1 and 2 and in low µg range for 3, 4 and 5 using calibration curves within 10-100 ng (R2 > 0.993). Recovery of spiked samples for all the recoveries observed were > 94%. Inter-day and intraday variations were 0.8-3.5% and 5-10.4%, respectively. These results indicate the suitability of the developed analytical method to detect and quantify triterpenes of raw materials used in the manufacture of natural health products |
| País: | Repositorio UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:null:11056/22332 |
| Acceso en línea: | http://hdl.handle.net/11056/22332 |
| Palabra clave: | PENTACYCLIC TRITERPENES QUALITY CONTROL SOUROUBEA HPLC-APCI-MS PLATANUS ANSIEDAD COMPORTAMIENTO ANIMAL |