Boid inclusion body disease is also a disease of wild Boa Constrictors
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| Autores: | , , , , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2022 |
| Descripción: | Reptarenaviruses cause boid inclusion body disease (BIBD), a poten- tially fatal disease, occurring in captive constrictor snakes boas and pythons worldwide. Classical BIBD, characterized by the formation of pathognomonic cyto- plasmic inclusion bodies (IBs), occurs mainly in boas, whereas in pythons, for example, reptarenavirus infection most often manifests as central nervous system signs with limited IB formation. The natural hosts of reptarenaviruses are unknown, although free-ranging/wild constrictor snakes are among the suspects. Here, we report BIBD with reptarenavirus infection in indigenous captive and wild boid snakes in Costa Rica using histology, immunohistology, transmission electron microscopy, and next-generation sequencing (NGS). The snakes studied represented diagnostic post- mortem cases of captive and wild-caught snakes since 1989. The results from NGS on archival paraffin blocks confirm that reptarenaviruses were already present in wild boa constrictors in Costa Rica in the 1980s. Continuous sequences that were de novo assembled from the low-quality RNA obtained from paraffin-embedded tissue allowed the identification of a distinct pair of reptarenavirus S and L segments in all studied animals; in most cases, reference assembly could recover almost complete segments. Sampling of three prospective cases in 2018 allowed an examination of fresh blood or tissues and resulted in the identification of additional reptarenavirus segments and hartmanivirus coinfection. Our results show that BIBD is not only a disease of captive snakes but also occurs in indigenous wild constrictor snakes in Costa Rica, suggesting boa constrictors to play a role in natural reptarenavirus circulation. |
| País: | Repositorio UNA |
| Institución: | Universidad Nacional de Costa Rica |
| Repositorio: | Repositorio UNA |
| Lenguaje: | Inglés |
| OAI Identifier: | oai:https://repositorio.una.ac.cr:11056/24320 |
| Acceso en línea: | http://hdl.handle.net/11056/24320 |
| Access Level: | acceso abierto |
| Palabra clave: | SERPIENTE SNAKE ORGANISMOS PATÓGENOS VIRAL PATHOGENESIS MICROBIOLOGÍA VETERINARIA VETERINARY MICROBIOLOGY ARENAVIRUS |