Screening of Latin American Plants for Cytotoxic Activity

 

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Bibliographic Details
Authors: Calderón, Ángela I., Vázquez, Yelkaira, Solís, Pablo N., Caballero George, Catherine, Zacchino, Susana Alicia, Gimenez, Alberto, Pinzón, Roberto, Cáceres, Armando, Tamayo Castillo, Giselle, Correa, Mireya, Gupta, Mahabir Prashad
Format: artículo original
Publication Date:2006
Description:The SRB cytotoxicity assay was used to screen plant extracts, in a collaborative multinational OAS project involving Argentina, Bolivia, Colombia, Costa Rica, Guatemala, Nicaragua and Panama, against breast (MCF-7), lung (H-460), and central nervous system (SF-268) human cancer cell lines. Out of 310 species tested, 23 (7.4%) plants showed cytotoxic activity at GI50 values ≤10 µg/ml. The most active plants were Thevetia ahouai., Physalis viscosa., Piper jacquemontianum., Piper barbatum., Senna occidentalis., Tovomita longifolia., and Lippia cardiostegia.. Blepharocalyx salicifolius. and Senna occidentalis. were selectively active against one cell line, SF-268 or MCF-7, respectively. Within the framework of this project, 14 compounds have been isolated, 5 new (4 benzophenones, coumarin) and 9 known to the literature. But only the bioassay-guided fractionation of the active extract of Piper barbatum. leaves, which led to the isolation of three known compounds: (2′E., 6′E.)-2-farnesyl-1,4-benzoquinone (1), (2′E., 6′E.)-2-farnesylhydroquinone (2), and dictyochromenol (3), is reported here. The chemical structures of 1 and 2 were determined by spectral means (1D, 2D NMR, MS) and chemical data. Among these three, (2′E., 6′E.)-2-farnesyl-1,4-benzoquinone was the most active (MCF-7 GI50 = 1.8 µg/ml; H-460 GI50 = 4.8 µg/ml; SF-268 GI50 = 3.5 µg/ml).
Country:Kérwá
Institution:Universidad de Costa Rica
Repositorio:Kérwá
Language:Inglés
OAI Identifier:oai:kerwa.ucr.ac.cr:10669/87506
Online Access:https://www.tandfonline.com/doi/full/10.1080/13880200600592285
https://hdl.handle.net/10669/87506
Keyword:Blepharocalyx salicifolius
Cytotoxicity
Latin American biodiversity
Piper barbatum
Senna occidentalis
SRB assay
Thevetia ahouai