Bactris guineensis (Arecaceae) extract: polyphenol characterization, antioxidant capacity and cytotoxicity against cancer cell lines
সংরক্ষণ করুন:
| লেখক: | , , , , , , , |
|---|---|
| বিন্যাস: | artículo original |
| প্রকাশনার তারিখ: | 2020 |
| বিবরন: | BACKGROUND:Lower risk of digestive tract cancer development has been associated with polyphenol intake. Bactris guineensis is an edible endemic palm that grows in Central and South America. OBJECTIVE:This study performs a phenolic characterization of Bactris guineensis and evaluates the bioactivity of this fruit. METHODS:The phenolic compounds of B. guineensis were characterized by HPLC-UV-HRMS analyses and the antioxidant activity was measured by chemical and cellular methods. Additionally, cytotoxicity of B. guineensis polyphenols was performed on 4 cancer cell lines and the pro-apoptotic effect was evaluated by flow cytometry using annexin staining. RESULTS:The major phenolic compounds of B. guineensis were proanthocyanidins. The extract IC50 for DPPH was 3.3±0.2 μg/mL and for induced intracellular ROS was 153±13 μg/mL. MTT cytotoxic assays demonstrate IC50 values between 16.6 and 24.9 μg/mL for the colon and hepatic adenocarcinomas, with high selectivity effects towards cancer cells compared to non-tumor cells. A 20 to 50% early apoptotic effect was observed in cancer cells lines by Annexin/PI staining. CONCLUSIONS:B. guineensis evidenced an important radical scavenging activity and a strong cytotoxic activity against hepatic and colorectal carcinoma cells, showing better values than procyanidin extracts from other fruits previously described. |
| দেশ: | Kérwá |
| প্রতিষ্ঠান: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| ভাষা: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/86128 |
| অনলাইন ব্যবহার করুন: | https://content.iospress.com/articles/journal-of-berry-research/jbr190449 https://hdl.handle.net/10669/86128 |
| মুখ্য শব্দ: | Bactris guineensis Antioxidant Polyphenols Apoptosis Cytotoxicity Procyanidins |