Exploring the antiangiogenic effects of Phospholipases A2 from Bothrops diporus venom
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| Autors: | , , , , , , , |
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| Format: | artículo original |
| Data de publicació: | 2025 |
| Descripció: | Angiogenesis, the formation of new blood vessels from pre-existing vasculature, is a crucial process in both physiologicaland pathological contexts, including cancer. Phospholipases A2(PLA2s), enzymes found in snake venoms, have attractedattention due to their potential antiangiogenic properties. In this study, we explored the antiangiogenic effects of PLA2isoforms isolated from Bothrops diporus venom using a combination of in vivo and ex vivo models. The chorioallantoicmembrane (CAM) assay revealed a significant reduction in vascular density and branching following PLA2streatment,with histological analysis confirming vascular regression, including vessel wall thinning and luminal collapse. Moreover,PLA2sinduced endothelial cell apoptosis, as shown by TUNEL staining, and reduced VEGF expression. The filter paperdisc variant of the CAM assay further supported these findings, demonstrating inhibited neovascularization while preservingmature vessels. Additionally, the CAM explant assay showed a marked decrease in vascular complexity and branching.These results demonstrate the antiangiogenic effect of PLA2isoforms from B. diporus and suggest that these enzymes maymodulate key angiogenic pathways. Based on our previous findings, this modulation may involve interference with integrinmediatedsignaling, which could underlie the vascular effects observed. Thus, this work provides compelling evidence forthe potential role of snake venom-derived PLA2sin modulating angiogenesis and highlights the need for further researchinto their mechanisms and possible biomedical applications. |
| Pais: | Kérwá |
| Institution: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| Idioma: | Inglés |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/104505 |
| Accés en línia: | https://doi.org/10.1007/s00441-025-04008-7 https://hdl.handle.net/10669/104505 |
| Paraula clau: | Phospholipase A2 Lysine-49 angiogenesis Bothrops diporus Chorioallantoic membrane (CAM) assay Apoptosis VEGF |