Myotoxin II from Bothrops asper (Terciopelo) venom is a lysine-49 phospholipase A2
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| Autores: | , , , |
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| Formato: | artículo original |
| Fecha de Publicación: | 1991 |
| Descripción: | A basic, dimeric myotoxic protein, myotoxin II, purified from Bothrops asper venom has a similar molecular weight and is immunologically cross-reactive with antibodies raised to previously isolated B. asper phospholipases A2, except that it shows only 0.1% of the phospholipase activity against L-alpha-phosphatidylcholine in the presence of Triton X-100. Its 121 amino acid sequence, determined by automated Edman degradation, clearly identifies it as a Lys-49 phospholipase A2. Key amino acid differences between myotoxin II and phospholipase active proteins in the Ca2(+)-binding loop region, include Lys for Asp-49, Asn for Tyr-28, and Leu for Gly-32. The latter substitution has not previously been seen in Lys-49 proteins. Other substitutions near the amino terminus (Leu for Phe-5 and Gln for several different amino acids at position 11) may prove useful for identifying other Lys-49 proteins in viperid and crotalid venoms. Myotoxin II shows greater sequence identity with other Lys-49 proteins from different snake venoms (Agkistrodon piscivorus piscivorus, Bothrops atrox, and Trimeresurus flavoviridis) than with another phospholipase A2 active Asp-49 molecule isolated from the same B. asper venom. This work demonstrates that phospholipase activity per se, is not required in phospholipase molecules for either myotoxicity or edema inducing activities. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/29165 |
| Acceso en línea: | http://www.sciencedirect.com/science/article/pii/0003986191903075 https://hdl.handle.net/10669/29165 |
| Palabra clave: | Amino Acid Sequence Animals Antibodies Calcium Cattle Cross Reactions Crotalid Venoms Group II Phospholipases A2 Humans Lysine Molecular Sequence Data Neurotoxins Octoxynol Phospholipases A Phospholipases A2 Polyethylene Glycols Protein Conformation Rats Reptilian Proteins Snake venom |