Production and partial characterization of monoclonal antibodies to Bothrops asper (terciopelo) myotoxin
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| Autores: | , |
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| Formato: | artículo original |
| Fecha de Publicación: | 1988 |
| Descripción: | Seven murine monoclonal antibodies against Bothrops aspen myotoxin were produced and partially characterized. They revealed the presence of at least four cross-reacting basic components in crude venom, with a common subunit mol. wt of 16,000 by sodium dodecylsulfate-polyacrylamide gel electrophoresis, but slight differences in charge. These myotoxin-related components might be isoforms of the toxin . By Western blotting and enzyme-immunoassay binding techniques, differences in the reactivities with basic venom fractions were observed among monoclonal antibodies, suggesting differences in epitope specifidties . Three antibodies cross-reacted with B. nummifer crude venom. Two monoclonal antibodies were utilized to develop a two-site enzyme-immunoassay for myotoxin detection at the nanogram level . Three of the antibodies (one IgM and two IgG,) showed ability to neutralize myotoxiclty of purified myotoxin in preincubation-type experiments. |
| País: | Kérwá |
| Institución: | Universidad de Costa Rica |
| Repositorio: | Kérwá |
| OAI Identifier: | oai:kerwa.ucr.ac.cr:10669/72949 |
| Acceso en línea: | http://www.sciencedirect.com/science/article/pii/0041010188902498 https://hdl.handle.net/10669/72949 |
| Palabra clave: | anticuerpos monoclonales miotoxina |